Hotspot

ycf3-trnS-GGA

LSC · 45139-46016 · 878 bp

Back to Celastrus

Pi 0.0158

MarkerSeek score 68.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 878
variable_sites: 53
pi: 0.0158
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1011-1102 bp	1.0	74.8
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1012-1103 bp	1.0	74.7
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	GGTGAAAGTGCGGAAAGAGA	45.0	50.0	56.4	58.1	1041-1132 bp	1.0	74.5
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	GGTGAAAGTGCGGAAAGAGA	45.0	50.0	56.4	58.1	1042-1133 bp	1.0	74.5
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	TGAAAGTGCGGAAAGAGAGG	45.0	50.0	56.4	57.8	1039-1130 bp	1.0	74.1