Hotspot

petN-psbM

LSC · 28870-29681 · 812 bp

Back to Celastrus

Pi 0.0192

MarkerSeek score 63.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 54
pi: 0.0192
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	CAGTCAAAGTCAGGGCGATA	50.0	50.0	57.6	57.3	835-912 bp	1.0	80.8
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TATCAGTCAAAGTCAGGGCG	50.0	50.0	57.6	57.3	838-915 bp	1.0	80.8
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	ATCAGTCAAAGTCAGGGCGA	50.0	50.0	57.6	59.0	837-914 bp	1.0	78.0
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TCAGTCAAAGTCAGGGCGAT	50.0	50.0	57.6	59.0	836-913 bp	1.0	78.0
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	CAGTCAAAGTCAGGGCGATAA	50.0	47.6	57.6	58.0	835-912 bp	1.0	78.0