Hotspot

atpH-atpI

LSC · 13330-14550 · 1221 bp

Back to Celastrus

Pi 0.0123

MarkerSeek score 69.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 63
pi: 0.0123
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATCACAGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1310-1348 bp	1.0	82.2
atpH-atpI_p2	ATCACAGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1309-1347 bp	1.0	82.1
atpH-atpI_p3	AATCACAGAAGCGGCAGAAA	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.1	57.4	1283-1321 bp	1.0	81.8
atpH-atpI_p4	ATCACAGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	57.9	57.4	1282-1320 bp	1.0	81.7
atpH-atpI_p5	AATAGAAGCAAGACCGACGG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.7	58.5	1343-1381 bp	1.0	80.5