Hotspot

rps16-trnQ-UUG

LSC · 5180-6143 · 964 bp

Back to Celastrus

Pi 0.0188

MarkerSeek score 69.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 964
variable_sites: 65
pi: 0.0188
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TTTCCTTCTCTTCGCGATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1080-1135 bp	1.0	87.4
rps16-trnQ-UUG_p2	TTTCCTTCTCTTCGCGATCG	AATAGAATATGGGGCGTGGC	50.0	50.0	58.1	57.8	1137-1192 bp	1.0	86.1
rps16-trnQ-UUG_p3	GCGATCGAACATCAATTGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.5	58.1	1067-1122 bp	1.0	84.5
rps16-trnQ-UUG_p4	GGTTTTCCTTCTCTTCGCGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1083-1138 bp	0.916667	82.7
rps16-trnQ-UUG_p5	GGTTTTCCTTCTCTTCGCGA	AATAGAATATGGGGCGTGGC	50.0	50.0	58.2	57.8	1140-1195 bp	0.916667	81.5