Hotspot

trnK-UUU-rps16

LSC · 4277-4885 · 609 bp

Back to Celastrus

Pi 0.0195
MarkerSeek score 65.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
609
variable_sites
49
pi
0.0195
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TCGGGGTTTTTACGCAATTC 50.0 45.0 58.2 57.0 690-723 bp 1.0 79.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TCGGGGTTTTTACGCAATTC 50.0 45.0 58.2 57.0 689-722 bp 1.0 79.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CGGGGTTTTTACGCAATTCT 50.0 45.0 58.2 57.0 689-722 bp 1.0 79.8
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CGGGGTTTTTACGCAATTCT 50.0 45.0 58.2 57.0 688-721 bp 1.0 79.8
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC TCGGGGTTTTTACGCAATTC 50.0 45.0 57.5 57.0 676-709 bp 1.0 77.7