Hotspot

trnH-GUG-psbA

LSC · 66-476 · 411 bp

Back to Celastrus

Pi 0.0471

MarkerSeek score 82.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 57
pi: 0.0471
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	521-576 bp	1.0	90.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	535-590 bp	1.0	90.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	534-589 bp	1.0	90.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	536-591 bp	1.0	90.7
trnH-GUG-psbA_p5	GATCCACAATCCACTGCCTT	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	541-596 bp	1.0	90.7