Hotspot

ycf1

SSC · 112185-117536 · 5352 bp

Back to Cedrela

Pi 0.352

MarkerSeek score 74.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5352
variable_sites: 1593
pi: 0.352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGAGACAGAACACGAAGGA	TTTCGGGGAGAACCAAAAGG	50.0	50.0	58.0	58.0	871 bp	0.5	59.9
ycf1_p2	AAGGACGTGTACCAGAGGAA	TTTCGGGGAGAACCAAAAGG	50.0	50.0	58.0	58.0	856 bp	0.5	59.9
ycf1_p3	AAATCGGATTTTCGGCGAGA	TTTCGGGGAGAACCAAAAGG	45.0	50.0	58.0	58.0	698 bp	0.5	59.8
ycf1_p4	AGGAGACAGAACACGAAGGA	AACGTTGTTTTCGGGGAGAA	50.0	45.0	58.0	58.0	879 bp	0.5	59.6
ycf1_p5	AAGGACGTGTACCAGAGGAA	AACGTTGTTTTCGGGGAGAA	50.0	45.0	58.0	58.0	864 bp	0.5	59.6