Hotspot

ndhI

SSC · 121744-122247 · 504 bp

Back to Cedrela

Pi 0.434

MarkerSeek score 80.20

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 197
pi: 0.434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GTTGATATAGTTGGGGCGCA	ACCCCATCACCAATAGTCCA	50.0	50.0	58.0	58.0	1686 bp	0.5	59.5
ndhI_p2	GTTGATATAGTTGGGGCGCA	TAAGTGGGAATTGATGCGGG	50.0	50.0	58.0	57.9	1274 bp	0.5	59.3
ndhI_p3	TCTTCCCAACTCCTTTCCCT	ACCCCATCACCAATAGTCCA	50.0	50.0	57.9	58.0	1191 bp	0.5	58.8
ndhI_p4	GTTGATATAGTTGGGGCGCA	AATACCACTCCCAGACCTCC	50.0	55.0	58.0	58.1	1467 bp	0.5	58.7
ndhI_p5	GTTGATATAGTTGGGGCGCA	CCCAAGGAAAAGGCCGAATA	50.0	50.0	58.0	57.9	1503 bp	0.5	58.6