Hotspot

ndhH

SSC · 118280-119476 · 1197 bp

Back to Cedrela

Pi 0.46

MarkerSeek score 79.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1197
variable_sites: 437
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AAGAAAACTGCGGATCGGTT	AAGAATTCCCAAAGGGCTGG	45.0	50.0	58.1	58.1	1746 bp	0.5	58.7
ndhH_p2	AAACTGCGGATCGGTTGAAT	AAGAATTCCCAAAGGGCTGG	45.0	50.0	58.2	58.1	1742 bp	0.5	58.1
ndhH_p3	AACTGCGGATCGGTTGAATT	AAGAATTCCCAAAGGGCTGG	45.0	50.0	58.2	58.1	1741 bp	0.5	58.1
ndhH_p4	ACTGCGGATCGGTTGAATTT	AAGAATTCCCAAAGGGCTGG	45.0	50.0	58.2	58.1	1740 bp	0.5	58.1
ndhH_p5	TGCGGATCGGTTGAATTTCA	AAGAATTCCCAAAGGGCTGG	45.0	50.0	58.2	58.1	1738 bp	0.5	58.1