Hotspot

ndhF

SSC · 129386-131614 · 2229 bp

Back to Cedrela

Pi 0.462

MarkerSeek score 79.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 884
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGGGAAAGGTATGAGTCATCC	GCAGAATACCGTCACCCATT	52.4	50.0	57.5	58.0	2951 bp	0.5	47.7
ndhF_p2	GGGGAAAGGTATGAGTCATCC	AGGGGGACTAAACAGGAACA	52.4	50.0	57.5	57.9	2856 bp	0.5	47.0
ndhF_p3	GGGGAAAGGTATGAGTCATCC	CGAGGAAACGGAAGAGATCC	52.4	55.0	57.5	57.8	2767 bp	0.5	46.4
ndhF_p4	GGGGAAAGGTATGAGTCATCC	ACAACTTTCGAAAGGAGGGG	52.4	50.0	57.5	57.7	2871 bp	0.5	45.9
ndhF_p5	GGGGAAAGGTATGAGTCATCC	AAGGAGGGGGACTAAACAGG	52.4	55.0	57.5	58.3	2860 bp	0.5	45.4