Hotspot

ccsA

SSC · 126072-127031 · 960 bp

Back to Cedrela

Pi 0.45

MarkerSeek score 81.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 382
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AATTGTGACCAGACGCCTTT	GTGAAATCGGTAGACACGCT	45.0	50.0	58.0	58.0	1293 bp	0.5	59.8
ccsA_p2	AATTGTGACCAGACGCCTTT	TGAAATCGGTAGACACGCTG	45.0	50.0	58.0	58.0	1292 bp	0.5	59.8
ccsA_p3	TTATCGATGGCTTCCCTTGC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1803 bp	0.5	59.7
ccsA_p4	TTATCGATGGCTTCCCTTGC	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1802 bp	0.5	59.7
ccsA_p5	TCTGGACCACGGGAGTTATT	GTGAAATCGGTAGACACGCT	50.0	50.0	58.1	58.0	1575 bp	0.5	59.5