Hotspot

rps15-ndhH

SSC · 118178-118279 · 102 bp

Back to Cedrela

Pi 0.293

MarkerSeek score 76.00

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 27
pi: 0.293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	TGGCAGTCGTTTCAGTATGG	GAAAGGAGTCTGTGCACCAA	50.0	50.0	57.9	58.0	1209 bp	0.5	59.0
rps15-ndhH_p2	GGCAGTCGTTTCAGTATGGT	GAAAGGAGTCTGTGCACCAA	50.0	50.0	57.9	58.0	1208 bp	0.5	59.0
rps15-ndhH_p3	TGGCAGTCGTTTCAGTATGG	CTATTCCAGACGCTCGTAGC	50.0	55.0	57.9	58.0	1504 bp	0.5	58.9
rps15-ndhH_p4	GGCAGTCGTTTCAGTATGGT	CTATTCCAGACGCTCGTAGC	50.0	55.0	57.9	58.0	1503 bp	0.5	58.9
rps15-ndhH_p5	TGGCAGTCGTTTCAGTATGG	CATGAGGTAAATCAGCCGCT	50.0	50.0	57.9	58.0	1324 bp	0.5	58.9