Hotspot

ycf1-rps15

SSC · 117537-117904 · 368 bp

Back to Cedrela

Pi 0.45

MarkerSeek score 77.00

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 368
variable_sites: 147
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TGGCAGTCGTTTCAGTATGG	ATGAGACGAAGAACACCGTG	50.0	50.0	57.9	57.9	902 bp	0.5	58.7
ycf1-rps15_p2	GGCAGTCGTTTCAGTATGGT	ATGAGACGAAGAACACCGTG	50.0	50.0	57.9	57.9	901 bp	0.5	58.7
ycf1-rps15_p3	TGGCAGTCGTTTCAGTATGG	AACCGATCCGCAGTTTTCTT	50.0	45.0	57.9	58.1	492 bp	0.5	58.4
ycf1-rps15_p4	GGCAGTCGTTTCAGTATGGT	AACCGATCCGCAGTTTTCTT	50.0	45.0	57.9	58.1	491 bp	0.5	58.4
ycf1-rps15_p5	TGGCAGTCGTTTCAGTATGG	CCAACGGGTTACATAAGGCA	50.0	50.0	57.9	57.9	1026 bp	0.5	58.3