Hotspot

petB

LSC · 79619-81077 · 1459 bp

Back to Cautleya

Pi 0.0172

MarkerSeek score 63.80

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1459
variable_sites: 25
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CCATTCTGGTAGTTCGACCG	TCTATCGTTCCTCGCCAGTA	55.0	50.0	58.1	57.7	1629-1662 bp	1.0	78.3
petB_p2	CCATTCTGGTAGTTCGACCG	ATTGTCTATCGTTCCTCGCC	55.0	50.0	58.1	57.5	1633-1666 bp	1.0	76.9
petB_p3	CCATTCTGGTAGTTCGACCG	ATCGTTCCTCGCCAGTATGA	55.0	50.0	58.1	58.6	1626-1659 bp	1.0	76.2
petB_p4	CCATTCTGGTAGTTCGACCG	TCGTTCCTCGCCAGTATGAT	55.0	50.0	58.1	58.6	1625-1658 bp	1.0	76.2
petB_p5	GGTAGTTCGACCGTGGAATT	TCTATCGTTCCTCGCCAGTA	50.0	50.0	57.6	57.7	1622-1655 bp	1.0	76.0