Hotspot

psaC-ndhE

SSC · 125957-126809 · 853 bp

Back to Cautleya

Pi 0.0157

MarkerSeek score 70.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 853
variable_sites: 12
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CGTCCCACGGTATCATTTCC	AACGCGTATCAACCAATCGA	55.0	45.0	58.4	58.0	896-987 bp	1.0	81.6
psaC-ndhE_p2	CGTCCCACGGTATCATTTCC	ACGCGTATCAACCAATCGAA	55.0	45.0	58.4	58.0	895-986 bp	1.0	81.6
psaC-ndhE_p3	CGTCCCACGGTATCATTTCC	CAACGCGTATCAACCAATCG	55.0	50.0	58.4	57.9	897-988 bp	1.0	81.1
psaC-ndhE_p4	GGCAAGCTCGTACACATTGA	AACGCGTATCAACCAATCGA	50.0	45.0	58.6	58.0	863-954 bp	1.0	80.6
psaC-ndhE_p5	GGCAAGCTCGTACACATTGA	ACGCGTATCAACCAATCGAA	50.0	45.0	58.6	58.0	862-953 bp	1.0	80.6