Hotspot

trnE-UUC-trnT-GGU

LSC · 33769-34629 · 861 bp

Back to Cautleya

Pi 0.00238

MarkerSeek score 62.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 861
variable_sites: 2
pi: 0.00238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TCCTTTCGTAGTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	979-1000 bp	1.0	80.7
trnE-UUC-trnT-GGU_p2	TCCTTTCGTAGTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	958-979 bp	1.0	80.2
trnE-UUC-trnT-GGU_p3	TCCTTTCGTAGTACCCCCAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.1	58.1	955-976 bp	1.0	80.1
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	943-964 bp	1.0	79.8
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	922-943 bp	1.0	79.7