Hotspot

trnS-GCU-trnG-UCC

LSC · 8798-9594 · 797 bp

Back to Cautleya

Pi 0.0246

MarkerSeek score 71.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 19
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CGGAGATACCCCTTCCCTTA	50.0	55.0	58.0	57.6	883-907 bp	1.0	80.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	CGGAGATACCCCTTCCCTTA	50.0	55.0	58.0	57.6	882-906 bp	1.0	80.7
trnS-GCU-trnG-UCC_p3	AGTATCAAGCGGGTCGAGTA	CGGAGATACCCCTTCCCTTA	50.0	55.0	58.0	57.6	967-991 bp	1.0	80.0
trnS-GCU-trnG-UCC_p4	GAGCGGAGAAAGAGGGATTC	CGGAGATACCCCTTCCCTTA	55.0	55.0	57.8	57.6	939-963 bp	1.0	78.6
trnS-GCU-trnG-UCC_p5	GTCGAGTAATCAGAGCGAGC	CGGAGATACCCCTTCCCTTA	55.0	55.0	58.3	57.6	955-979 bp	1.0	78.4