Hotspot

psaC

SSC · 122753-122998 · 246 bp

Back to Caulophyllum

Pi 0.487

MarkerSeek score 76.80

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 111
pi: 0.487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TCGCATGAGCAAATCCAGAA	ACAAACGTTTCTTCCCTCCC	45.0	50.0	57.9	58.0	1028 bp	0.5	58.9
psaC_p2	CCGAAGCGGCTATTGGATTA	ACAAACGTTTCTTCCCTCCC	50.0	50.0	57.8	58.0	1249 bp	0.5	58.5
psaC_p3	TCGCATGAGCAAATCCAGAA	TTTGACCGCTGTACATTGCT	45.0	45.0	57.9	58.1	920 bp	0.5	58.2
psaC_p4	TCGCATGAGCAAATCCAGAA	CCCCCGCAGTGTACAAAATA	45.0	50.0	57.9	57.9	1064 bp	0.5	58.2
psaC_p5	TCGCATGAGCAAATCCAGAA	TCAAATGGATGCAGGTGGAG	45.0	50.0	57.9	57.9	428 bp	0.5	58.0