Hotspot

ndhI

SSC · 120301-120852 · 552 bp

Back to Caulophyllum

Pi 0.46

MarkerSeek score 80.60

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 552
variable_sites: 223
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TTTTGGGGGTGGAATCTGTG	GCTGCGATTCTCACAACCTA	50.0	50.0	58.0	58.0	1206 bp	0.5	59.9
ndhI_p2	GGTTTTTGGGGGTGGAATCT	GCTGCGATTCTCACAACCTA	50.0	50.0	58.0	58.0	1209 bp	0.5	59.7
ndhI_p3	GGAATCTCGCTATTCCGGAC	GCTGCGATTCTCACAACCTA	55.0	50.0	57.9	58.0	977 bp	0.5	59.4
ndhI_p4	ACAGTTCTTTACTTGGGCGG	GCTGCGATTCTCACAACCTA	50.0	50.0	58.1	58.0	999 bp	0.5	59.1
ndhI_p5	CAGTTCTTTACTTGGGCGGT	GCTGCGATTCTCACAACCTA	50.0	50.0	58.1	58.0	998 bp	0.5	59.1