Hotspot

ndhF

SSC · 128704-130932 · 2229 bp

Back to Caulophyllum

Pi 0.472

MarkerSeek score 79.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 905
pi: 0.472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTCGTAGCGTTTCCCGTAAA	CCGGGTGAATGGAAAGGAAA	50.0	50.0	58.0	58.1	2977 bp	0.5	59.2
ndhF_p2	ATTATTGGCGATTGGCGGAT	ACGAAGGGGACTAAACAGGA	45.0	50.0	58.1	58.0	2942 bp	0.5	59.1
ndhF_p3	CTCGTAGCGTTTCCCGTAAA	AAGAGATCCGGGTGAATGGA	50.0	50.0	58.0	58.1	2984 bp	0.5	58.9
ndhF_p4	CTCGTAGCGTTTCCCGTAAA	AGAGATCCGGGTGAATGGAA	50.0	50.0	58.0	58.1	2983 bp	0.5	58.9
ndhF_p5	ATTATTGGCGATTGGCGGAT	CCTTCCCCTTCCCTTTGTTC	45.0	55.0	58.1	58.1	2897 bp	0.5	58.7