Hotspot

ndhE

SSC · 122172-122477 · 306 bp

Back to Caulophyllum

Pi 0.47

MarkerSeek score 76.40

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 131
pi: 0.47
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GGGGTCAACAACTTGGGATT	TGCTACGAGTTGTCTCATGC	50.0	50.0	58.0	58.0	1163 bp	0.5	59.9
ndhE_p2	GGGGTCAACAACTTGGGATT	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	58.0	58.0	1074 bp	0.5	59.8
ndhE_p3	GGGGTCAACAACTTGGGATT	CCCATGCTACGAGTTGTCTC	50.0	55.0	58.0	58.1	1167 bp	0.5	59.3
ndhE_p4	TTTTGGACCGTTGGAGATGG	TGCTACGAGTTGTCTCATGC	50.0	50.0	58.1	58.0	1308 bp	0.5	59.2
ndhE_p5	GGGGTCAACAACTTGGGATT	GTCCTCTGTTCTTGGAGCAG	50.0	55.0	58.0	57.9	1075 bp	0.5	59.2