Hotspot

ndhD

SSC · 123151-124683 · 1533 bp

Back to Caulophyllum

Pi 0.493

MarkerSeek score 82.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1533
variable_sites: 678
pi: 0.493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCATGAGACAACTCGTAGCA	GCAAGGCGTAAATTCTGCAA	50.0	45.0	58.0	57.7	2400 bp	0.5	57.2
ndhD_p2	GCATGAGACAACTCGTAGCA	TTGCAAGGCGTAAATTCTGC	50.0	45.0	58.0	57.7	2402 bp	0.5	57.2
ndhD_p3	TCTGCTCCAAGAACAGAGGA	GCAAGGCGTAAATTCTGCAA	50.0	45.0	58.0	57.7	2489 bp	0.5	57.1
ndhD_p4	TCTGCTCCAAGAACAGAGGA	TTGCAAGGCGTAAATTCTGC	50.0	45.0	58.0	57.7	2491 bp	0.5	57.1
ndhD_p5	GAGACAACTCGTAGCATGGG	GCAAGGCGTAAATTCTGCAA	55.0	45.0	58.1	57.7	2396 bp	0.5	56.7