Hotspot

ndhA

SSC · 118058-120222 · 2165 bp

Back to Caulophyllum

Pi 0.476

MarkerSeek score 78.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2165
variable_sites: 834
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTGAGAGTAGAAGCCCCAA	TGACCAATATACCTTGCGGC	50.0	50.0	58.0	58.0	2517 bp	0.5	59.5
ndhA_p2	TGTGAGAGTAGAAGCCCCAA	CTCGGACTGTTTGTTGACCA	50.0	50.0	58.0	58.1	2498 bp	0.5	59.4
ndhA_p3	TGTGAGAGTAGAAGCCCCAA	GGCATACACGAACACAGACT	50.0	50.0	58.0	57.9	2669 bp	0.5	59.2
ndhA_p4	AAATTGGGGCTTATCCGGAC	TGACCAATATACCTTGCGGC	50.0	50.0	57.9	58.0	2853 bp	0.5	59.2
ndhA_p5	AAATTGGGGCTTATCCGGAC	CTCGGACTGTTTGTTGACCA	50.0	50.0	57.9	58.1	2834 bp	0.5	59.0