Hotspot

rpl32-ndhF

SSC · 127562-128703 · 1142 bp

Back to Caulophyllum

Pi 0.456

MarkerSeek score 82.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1142
variable_sites: 468
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AGTACGTTTCTTCGGAACCG	ACAATTGGTCGTGTGAACCA	50.0	45.0	57.9	58.0	1678 bp	0.5	59.2
rpl32-ndhF_p2	AGTACGTTTCTTCGGAACCG	CGAATAAGAGGGAGGCACAC	50.0	55.0	57.9	58.1	1869 bp	0.5	59.1
rpl32-ndhF_p3	AGTACGTTTCTTCGGAACCG	AGGTACGAGCACATTCCAAC	50.0	50.0	57.9	57.9	1645 bp	0.5	58.8
rpl32-ndhF_p4	AGTACGTTTCTTCGGAACCG	GGTACGAGCACATTCCAACT	50.0	50.0	57.9	57.9	1644 bp	0.5	58.8
rpl32-ndhF_p5	AGTACGTTTCTTCGGAACCG	AAGCCCCACTCCTATTGCTA	50.0	50.0	57.9	58.1	1244 bp	0.5	58.7