Hotspot

ccsA-trnL-UAG

SSC · 126207-126305 · 99 bp

Back to Caulophyllum

Pi 0.367

MarkerSeek score 76.20

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: SSC
length_bp: 99
variable_sites: 33
pi: 0.367
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	ATTCACGTCTTGGATCTCGC	TTCTCTGCCGGGACTAAGAA	50.0	50.0	58.1	58.1	1018 bp	0.5	58.9
ccsA-trnL-UAG_p2	ATTCACGTCTTGGATCTCGC	GAACAGAACTCTCCGGTTCC	50.0	55.0	58.1	57.9	1001 bp	0.5	58.8
ccsA-trnL-UAG_p3	AAGTAGCAAAGCCTTGGGTC	TTCTCTGCCGGGACTAAGAA	50.0	50.0	58.1	58.1	779 bp	0.5	58.8
ccsA-trnL-UAG_p4	GAAGTAGCAAAGCCTTGGGT	TTCTCTGCCGGGACTAAGAA	50.0	50.0	58.1	58.1	780 bp	0.5	58.8
ccsA-trnL-UAG_p5	TGAAGTAGCAAAGCCTTGGG	TTCTCTGCCGGGACTAAGAA	50.0	50.0	58.1	58.1	781 bp	0.5	58.7