Hotspot

psbT-psbN

LSC · 75118-75191 · 74 bp

Back to Caulophyllum

Pi 0.108

MarkerSeek score 87.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 74
variable_sites: 8
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTCGAGAACCACCTAAGGT	ACCCTAGTCGCCATCTTTCT	45.0	50.0	56.1	58.1	236 bp	1.0	66.4
psbT-psbN_p2	TTTCGAGAACCACCTAAGGT	GATCCATTCGAGGAACACGG	45.0	55.0	56.1	58.4	143 bp	1.0	65.8
psbT-psbN_p3	TTTTCGAGAACCACCTAAGGT	ACCCTAGTCGCCATCTTTCT	42.9	50.0	56.9	58.1	237 bp	1.0	64.6
psbT-psbN_p4	TTTCGAGAACCACCTAAGGT	GGCAACCCTCTCAACAACTA	45.0	50.0	56.1	57.4	166 bp	1.0	64.1
psbT-psbN_p5	AGGTTCCAACTAAAAGGGTCA	ACCCTAGTCGCCATCTTTCT	42.9	50.0	56.8	58.1	220 bp	1.0	63.9