Hotspot

rps19

LSC · 147948-148226 · 279 bp

Back to Cattleya

Pi 0.043

MarkerSeek score 69.30

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 12
pi: 0.043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	AAAAGGAGGAGTAATCAGCC	AGGCTTTTCACATGAATACT	45.0	35.0	54.7	52.4	330-333 bp	1.0	42.9
rps19_p2	AAAAGGAGGAGTAATCAGCC	AAGGCTTTTCACATGAATACT	45.0	33.3	54.7	53.4	331-334 bp	1.0	42.9
rps19_p3	AAAAGGAGGAGTAATCAGCC	AGGCTTTTCACATGAATACTT	45.0	33.3	54.7	53.4	330-333 bp	1.0	42.9
rps19_p4	AAAAGGAGGAGTAATCAGCC	AGGCTTTTCACATGAATACTTAGT	45.0	33.3	54.7	56.4	330-333 bp	1.0	42.9
rps19_p5	AAAAAGGAGGAGTAATCAGCC	AGGCTTTTCACATGAATACT	42.9	35.0	55.6	52.4	331-334 bp	1.0	42.9