Hotspot

ndhK

LSC · 49570-50297 · 728 bp

Back to Cattleya

Pi 0.00412

MarkerSeek score 61.10

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 728
variable_sites: 3
pi: 0.00412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TCTTTACGTAAAGGCCAGCC	GGGGATGCTTGGGTACAATT	50.0	50.0	57.9	57.8	845 bp	1.0	78.2
ndhK_p2	CGACACGACTCCAGATTTCA	GGGGATGCTTGGGTACAATT	50.0	50.0	57.7	57.8	920 bp	1.0	76.2
ndhK_p3	ACGACACGACTCCAGATTTC	GGGGATGCTTGGGTACAATT	50.0	50.0	57.7	57.8	921 bp	1.0	76.2
ndhK_p4	TCTTTACGTAAAGGCCAGCC	TGCTTGGGTACAATTCCGAA	50.0	45.0	57.9	57.4	840 bp	1.0	74.9
ndhK_p5	TCTTTACGTAAAGGCCAGCC	GGGTACAATTCCGAATTCGC	50.0	50.0	57.9	57.2	835 bp	1.0	73.5