Hotspot

ndhE

SSC · 115768-116073 · 306 bp

Back to Cattleya

Pi 0.00654

MarkerSeek score 52.60

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 2
pi: 0.00654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GGACAAGCGGATTCACATCT	ATGGTGTTCTTCGGCTGATC	50.0	50.0	58.0	58.0	1330-1397 bp	1.0	83.8
ndhE_p2	AATACATCCGTGGGACAAGC	ATGGTGTTCTTCGGCTGATC	50.0	50.0	58.0	58.0	1231-1298 bp	1.0	83.8
ndhE_p3	AATACATCCGTGGGACAAGC	ACCCATCAATGCATGGTGTT	50.0	45.0	58.0	58.1	1243-1310 bp	1.0	83.6
ndhE_p4	GGACAAGCGGATTCACATCT	ACCCATCAATGCATGGTGTT	50.0	45.0	58.0	58.1	1342-1409 bp	1.0	83.5
ndhE_p5	GGACAAGCGGATTCACATCT	TGACTGTACCGGCTACAAGA	50.0	50.0	58.0	58.1	1396-1463 bp	1.0	83.2