Hotspot

ndhD

SSC · 113400-114904 · 1505 bp

Back to Cattleya

Pi 0.00532

MarkerSeek score 55.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1505
variable_sites: 8
pi: 0.00532
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGTACAACGAAGAATAGTGAGA	CGACAAAAGCCTGTACTCGA	36.4	50.0	54.6	57.9	1692-1695 bp	1.0	40.4
ndhD_p2	TGTACAACGAAGAATAGTGAGA	ACCGACAAAAGCCTGTACTC	36.4	50.0	54.6	57.8	1694-1697 bp	1.0	40.4
ndhD_p3	TGTACAACGAAGAATAGTGAGA	CACGGGCTTTTCTGGTCTAA	36.4	50.0	54.6	57.8	1642-1645 bp	1.0	40.4
ndhD_p4	TGTACAACGAAGAATAGTGAGAA	CGACAAAAGCCTGTACTCGA	34.8	50.0	55.4	57.9	1692-1695 bp	1.0	40.4
ndhD_p5	TTGTACAACGAAGAATAGTGAGA	CGACAAAAGCCTGTACTCGA	34.8	50.0	55.4	57.9	1693-1696 bp	1.0	40.4