Hotspot

ndhC

LSC · 50180-50539 · 360 bp

Back to Cattleya

Pi 0
MarkerSeek score 31.60
Primer available yes
Label ndhC

Feature Metadata

label_name
ndhC
feature_type
gene
region
LSC
length_bp
360
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC_p1 TGAATTGGGGGTTGTTTGGT TTCAGCCTACTCCACATTCA 45.0 45.0 57.8 56.2 500 bp 1.0 63.8
ndhC_p2 TGAATTGGGGGTTGTTTGGT CAGCCTACTCCACATTCATAGG 45.0 50.0 57.8 58.0 498 bp 1.0 62.5
ndhC_p3 TGAATTGGGGGTTGTTTGGT AGCCTACTCCACATTCATAGGA 45.0 45.5 57.8 58.1 497 bp 1.0 62.0
ndhC_p4 TGAATTGGGGGTTGTTTGGT TCAGCCTACTCCACATTCAT 45.0 45.0 57.8 55.9 499 bp 1.0 61.9
ndhC_p5 TGAATTGGGGGTTGTTTGGT TTCAGCCTACTCCACATTCAT 45.0 42.9 57.8 56.7 500 bp 1.0 60.2