Hotspot

clpP

LSC · 70718-72960 · 2243 bp

Back to Cattleya

Pi 0.000892
MarkerSeek score 51.70
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2243
variable_sites
2
pi
0.000892
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 GCGGGTTGGTTTAGATCGAT AAACCATTGTTACGTTTCCA 50.0 35.0 58.1 53.6 2410-2412 bp 1.0 44.6
clpP_p2 GCGGGTTGGTTTAGATCGAT AAAACCATTGTTACGTTTCCA 50.0 33.3 58.1 54.5 2411-2413 bp 1.0 43.7
clpP_p3 GCGGGTTGGTTTAGATCGAT AACCATTGTTACGTTTCCAT 50.0 35.0 58.1 53.4 2409-2411 bp 1.0 42.7
clpP_p4 GCGGGTTGGTTTAGATCGAT AAACCATTGTTACGTTTCCAT 50.0 33.3 58.1 54.3 2410-2412 bp 1.0 41.9
clpP_p5 GCGGGTTGGTTTAGATCGAT AAAACCATTGTTACGTTTCCAT 50.0 31.8 58.1 55.1 2411-2413 bp 1.0 40.4