Hotspot

ycf1-trnN-GUU

SSC · 123626-123965 · 340 bp

Back to Cattleya

Pi 0

MarkerSeek score 26.50

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: SSC
length_bp: 340
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 26.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	TAGTGAAAATAGGCGACGGC	GGCAGAATAGCAGAGCAAGT	50.0	50.0	58.1	58.0	1105-1459 bp	1.0	85.3
ycf1-trnN-GUU_p2	TAGTGAAAATAGGCGACGGC	GTAACCTGGGCTGGAATCAG	50.0	55.0	58.1	58.0	1170-1524 bp	1.0	85.1
ycf1-trnN-GUU_p3	CCTTCTTCCATAACCCGAGC	GGCAGAATAGCAGAGCAAGT	55.0	50.0	58.0	58.0	905 bp	0.5	59.4
ycf1-trnN-GUU_p4	CCTTCTTCCATAACCCGAGC	GTAACCTGGGCTGGAATCAG	55.0	55.0	58.0	58.0	970 bp	0.5	59.3
ycf1-trnN-GUU_p5	CCTTCTTCCATAACCCGAGC	AAGCGAGCCCTTTCATTCAA	55.0	45.0	58.0	58.1	622 bp	0.5	58.9