Hotspot

ndhE-ndhG

SSC · 116074-116269 · 196 bp

Back to Cattleya

Pi 0.0217

MarkerSeek score 67.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 196
variable_sites: 4
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	CCATGCTTTGGCTTGTGATG	AGGTGCAATTACTATGGCTCG	50.0	47.6	58.0	58.2	291-303 bp	1.0	75.0
ndhE-ndhG_p2	TGCTTTGGCTTGTGATGAGT	AGGTGCAATTACTATGGCTCG	45.0	47.6	58.0	58.2	288-300 bp	1.0	74.9
ndhE-ndhG_p3	CCATGCTTTGGCTTGTGATG	GCCTTATGTAACACGTTGGG	50.0	50.0	58.0	56.8	379-391 bp	1.0	73.7
ndhE-ndhG_p4	TGCTTTGGCTTGTGATGAGT	GCCTTATGTAACACGTTGGG	45.0	50.0	58.0	56.8	376-388 bp	1.0	73.7
ndhE-ndhG_p5	CCATGCTTTGGCTTGTGATG	GCCTTATGTAACACGTTGGGA	50.0	47.6	58.0	58.3	379-391 bp	1.0	72.9