Hotspot

rbcL-accD

LSC · 56604-57461 · 858 bp

Back to Cattleya

Pi 0.0012

MarkerSeek score 62.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 858
variable_sites: 1
pi: 0.0012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	CCACTGGTATGTCCAAGAGC	55.0	55.0	58.0	58.0	1042-1066 bp	1.0	81.4
rbcL-accD_p2	TGAAGCTAGCAAATGGAGCC	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.2	58.0	1047-1071 bp	1.0	79.7
rbcL-accD_p3	CGCTGCTTGTGAAGTATGGA	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.3	58.0	1017-1041 bp	1.0	79.5
rbcL-accD_p4	TTTCGACCCAGTGGATAAGC	CCACTGGTATGTCCAAGAGC	50.0	55.0	57.6	58.0	981-1005 bp	1.0	78.6
rbcL-accD_p5	TTCGACCCAGTGGATAAGCT	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.4	58.0	980-1004 bp	1.0	78.3