Hotspot

atpB-rbcL

LSC · 54182-55139 · 958 bp

Back to Cattleya

Pi 0.00744

MarkerSeek score 64.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 958
variable_sites: 7
pi: 0.00744
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGCCCGGTGGAAAAATTACA	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.0	58.1	1085-1102 bp	1.0	80.8
atpB-rbcL_p2	ATCTTGCCCGGTGGAAAAAT	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.1	58.1	1089-1106 bp	1.0	80.3
atpB-rbcL_p3	TGAGTGATACGCCCTAGGTT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	1044-1061 bp	1.0	79.6
atpB-rbcL_p4	TTGAGTGATACGCCCTAGGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	1045-1062 bp	1.0	79.6
atpB-rbcL_p5	TCTTGCCCGGTGGAAAAATT	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.3	58.1	1088-1105 bp	1.0	78.5