Hotspot

ndhC-trnV-UAC

LSC · 50540-51177 · 638 bp

Back to Cattleya

Pi 0.00627

MarkerSeek score 62.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 4
pi: 0.00627
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAATCCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	770-776 bp	1.0	77.7
ndhC-trnV-UAC_p2	TCTGGACCTTCCCTAATCGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	787-793 bp	1.0	77.1
ndhC-trnV-UAC_p3	AATCGGGGCTAAAATCCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	773-779 bp	1.0	75.9
ndhC-trnV-UAC_p4	CGGGGCTAAAATCCCAGAAA	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	852-858 bp	1.0	75.5
ndhC-trnV-UAC_p5	CGGGGCTAAAATCCCAGAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	769-775 bp	1.0	75.0