Hotspot

rps16-trnQ-UUG

LSC · 5327-7201 · 1875 bp

Back to Cattleya

Pi 0.0011

MarkerSeek score 61.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1875
variable_sites: 2
pi: 0.0011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TTCCTTCTCTTCGGGATCGA	GACCAAATCAAAATGGGGCG	50.0	50.0	57.9	58.0	2018-2070 bp	1.0	81.0
rps16-trnQ-UUG_p2	TTCCTTCTCTTCGGGATCGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1957-2009 bp	1.0	80.5
rps16-trnQ-UUG_p3	GATGCCCCCGAAACGTATAG	GACCAAATCAAAATGGGGCG	55.0	50.0	58.2	58.0	1945-1997 bp	1.0	80.5
rps16-trnQ-UUG_p4	GATGCCCCCGAAACGTATAG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.2	58.1	1884-1936 bp	1.0	80.1
rps16-trnQ-UUG_p5	TTCCTTCTCTTCGGGATCGA	CTCGGAGGTTCGAATCCTTC	50.0	55.0	57.9	57.8	1961-2013 bp	1.0	79.4