Hotspot

ycf1

SSC · 10736-16336 · 5601 bp

Back to Catopsis

Pi 0.00488

MarkerSeek score 40.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5601
variable_sites: 41
pi: 0.00488
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTTTCCATTTTCAGCCGT	GGAGTCCCCGAAAAAGACTT	40.0	50.0	55.9	57.4	241 bp	1.0	60.0
ycf1_p2	TCTTTTCCATTTTCAGCCGTT	GGAGTCCCCGAAAAAGACTT	38.1	50.0	56.6	57.4	241 bp	1.0	58.2
ycf1_p3	ATCTTTTCCATTTTCAGCCGT	GGAGTCCCCGAAAAAGACTT	38.1	50.0	56.4	57.4	242 bp	1.0	56.4
ycf1_p4	TCTTTTCCATTTTCAGCCGTTT	GGAGTCCCCGAAAAAGACTT	36.4	50.0	57.3	57.4	241 bp	1.0	55.8
ycf1_p5	TCTTTTCCATTTTCAGCCGT	GTCCCCGAAAAAGACTTTCCT	40.0	47.6	55.9	58.1	238 bp	1.0	55.4