Hotspot

psbE-petL

LSC · 108795-110267 · 1473 bp

Back to Catopsis

Pi 0.00861

MarkerSeek score 57.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1473
variable_sites: 19
pi: 0.00861
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1620-1705 bp	1.0	76.8
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1618-1703 bp	1.0	76.8
psbE-petL_p3	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1622-1707 bp	1.0	75.3
psbE-petL_p4	ACCAACCCGCAATGAATAGG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	58.2	58.5	1620-1705 bp	1.0	75.3
psbE-petL_p5	CCGCAATGAATAGGGAAGGT	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.7	58.5	1616-1701 bp	1.0	74.4