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accD-psaI

LSC · 102337-103289 · 953 bp

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Pi 0.0136

MarkerSeek score 69.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 19
pi: 0.0136
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1049-1067 bp	1.0	83.1
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1048-1066 bp	1.0	83.1
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1055-1073 bp	1.0	82.4
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1054-1072 bp	1.0	82.4
accD-psaI_p5	GACCCAATCGTACCACGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	1101-1119 bp	1.0	79.4