Hotspot

ndhC-trnV-UAC

LSC · 93970-94995 · 1026 bp

Back to Catopsis

Pi 0.00715

MarkerSeek score 61.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1026
variable_sites: 11
pi: 0.00715
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1159-1183 bp	1.0	79.2
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAACTCC	GAGCCCAATGCAACAAACAA	55.0	45.0	58.0	57.8	1246-1270 bp	1.0	78.8
ndhC-trnV-UAC_p3	CGGGGCTAAAACTCCTGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1154-1178 bp	1.0	77.9
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCTGAAA	GAGCCCAATGCAACAAACAA	50.0	45.0	57.8	57.8	1241-1265 bp	1.0	77.5
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAACTCCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1158-1182 bp	1.0	76.7