Hotspot

trnT-UGU-trnL-UAA

LSC · 89732-90512 · 781 bp

Back to Catopsis

Pi 0.00854
MarkerSeek score 55.80
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
781
variable_sites
10
pi
0.00854
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 831-841 bp 1.0 80.4
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC ACCAATTTCGCCATATCCCC 55.0 50.0 58.2 58.0 862-872 bp 1.0 80.2
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 839-849 bp 1.0 79.9
trnT-UGU-trnL-UAA_p4 GAGTCGAACCGATGACCATC TAGCGTCTACCAATTTCGCC 55.0 50.0 58.2 58.1 870-880 bp 1.0 79.6
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG ATCCAATCAAGTCCGTAGCG 55.0 50.0 57.9 57.8 854-864 bp 1.0 78.6