Hotspot

trnS-GCU-trnG-UCC

LSC · 49864-51262 · 1399 bp

Back to Catopsis

Pi 0.00878

MarkerSeek score 59.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1399
variable_sites: 17
pi: 0.00878
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	AACCGATGGACCCCTTAACT	45.0	50.0	58.0	58.3	1474-1511 bp	1.0	80.7
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	AACCGATGGACCCCTTAACT	50.0	50.0	58.2	58.3	1523-1560 bp	1.0	79.7
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AAACCGATGGACCCCTTAAC	45.0	50.0	58.0	57.5	1475-1512 bp	1.0	79.4
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	ACCGATGGACCCCTTAACTA	45.0	50.0	58.0	57.4	1473-1510 bp	1.0	79.0
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	AAACCGATGGACCCCTTAAC	50.0	50.0	58.2	57.5	1524-1561 bp	1.0	78.4