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rps15-ycf1

SSC · 10337-10735 · 399 bp

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Pi 0.0217

MarkerSeek score 55.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 399
variable_sites: 13
pi: 0.0217
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	CTCCTAGGCGTCGTAGAGAA	45.0	55.0	58.1	58.0	1153-1154 bp	1.0	80.9
rps15-ycf1_p2	AATAAGCCAGCAACCGTTGA	CTCCTAGGCGTCGTAGAGAA	45.0	55.0	58.1	58.0	906 bp	1.0	80.4
rps15-ycf1_p3	AACACCATGCATTGATGGGT	ATCTCCTAGGCGTCGTAGAG	45.0	55.0	58.1	57.8	1155-1156 bp	1.0	79.9
rps15-ycf1_p4	AATAAGCCAGCAACCGTTGA	ATCTCCTAGGCGTCGTAGAG	45.0	55.0	58.1	57.8	908 bp	1.0	79.4
rps15-ycf1_p5	ATTAGCGGGTTTTTGGCTCT	CTCCTAGGCGTCGTAGAGAA	45.0	55.0	57.8	58.0	981 bp	1.0	79.3