Hotspot

ndhH-rps15

SSC · 9966-10063 · 98 bp

Back to Catopsis

Pi 0.0544
MarkerSeek score 64.20
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
98
variable_sites
8
pi
0.0544
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 AACACCATGCATTGATGGGT TCAACGGTTGCTGGCTTATT 45.0 45.0 58.1 58.1 267-268 bp 1.0 81.2
ndhH-rps15_p2 AACACCATGCATTGATGGGT CGGTTGCTGGCTTATTTGTC 45.0 50.0 58.1 57.7 263-264 bp 1.0 79.9
ndhH-rps15_p3 ATGCATTGATGGGTGGTGAG TCAACGGTTGCTGGCTTATT 50.0 45.0 58.2 58.1 261-262 bp 1.0 79.9
ndhH-rps15_p4 AACACCATGCATTGATGGGT ACGGTTGCTGGCTTATTTGT 45.0 45.0 58.1 58.4 264-265 bp 1.0 78.9
ndhH-rps15_p5 ATGCATTGATGGGTGGTGAG CGGTTGCTGGCTTATTTGTC 50.0 50.0 58.2 57.7 257-258 bp 1.0 78.6