Hotspot

ccsA-ndhD

SSC · 2027-2291 · 265 bp

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Pi 0.0528
MarkerSeek score 73.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
265
variable_sites
21
pi
0.0528
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GGAAAGGTACGAATTCGGCA CAAGGTACATGCTATCCTATCCA 50.0 43.5 58.3 57.3 421 bp 1.0 51.9
ccsA-ndhD_p2 TGGAAAGGTACGAATTCGGC CAAGGTACATGCTATCCTATCCA 50.0 43.5 58.3 57.3 422 bp 1.0 51.9
ccsA-ndhD_p3 GGAAAGGTACGAATTCGGCA ACAAGGTACATGCTATCCTATCC 50.0 43.5 58.3 57.3 422 bp 1.0 51.8
ccsA-ndhD_p4 TGGAAAGGTACGAATTCGGC ACAAGGTACATGCTATCCTATCC 50.0 43.5 58.3 57.3 423 bp 1.0 51.8
ccsA-ndhD_p5 AGGTACGAATTCGGCACTTG CAAGGTACATGCTATCCTATCCA 50.0 43.5 58.3 57.3 417 bp 1.0 51.8