Hotspot

ycf1

SSC · 124740-130415 · 5676 bp

Back to Cathetus

Pi 0.0589

MarkerSeek score 70.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5676
variable_sites: 324
pi: 0.0589
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGCGATTCCAGCGTTTATA	GCTGTTAGTCTGGATCCAGC	50.0	55.0	58.1	58.1	579 bp	0.5	58.6
ycf1_p2	TCCACGATAAGGCCCACTTA	GCTGTTAGTCTGGATCCAGC	50.0	55.0	58.1	58.1	973 bp	0.5	58.5
ycf1_p3	TTTTCCACGATAAGGCCCAC	GCTGTTAGTCTGGATCCAGC	50.0	55.0	58.2	58.1	976 bp	0.5	58.2
ycf1_p4	GGGCGATTCCAGCGTTTATA	TCCTAGCGCTACCGTATCTT	50.0	50.0	58.1	57.7	862 bp	0.5	56.9
ycf1_p5	TCCACGATAAGGCCCACTTA	TCCTAGCGCTACCGTATCTT	50.0	50.0	58.1	57.7	1256 bp	0.5	56.8