Hotspot

ndhF-rpl32

SSC · 113445-114381 · 937 bp

Back to Cathetus

Pi 0.0623
MarkerSeek score 64.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
937
variable_sites
22
pi
0.0623
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.38
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCATGAATATTGATATGTATGTTCCA GTACGTTTCTTTGGAACTGCC 29.6 47.6 56.7 57.7 407-988 bp 1.0 46.5
ndhF-rpl32_p2 CCATGAATATTGATATGTATGTTCCA GTACGTTTCTTTGGAACTGCC 30.8 47.6 55.5 57.7 406-987 bp 1.0 46.5
ndhF-rpl32_p3 TCCATGAATATTGATATGTATGTTCCA TACGTTTCTTTGGAACTGCC 29.6 45.0 56.7 56.3 406-987 bp 1.0 46.5
ndhF-rpl32_p4 TCCATGAATATTGATATGTATGTTCC GTACGTTTCTTTGGAACTGCC 30.8 47.6 55.3 57.7 407-988 bp 1.0 46.5
ndhF-rpl32_p5 CCATGAATATTGATATGTATGTTCCA TACGTTTCTTTGGAACTGCC 30.8 45.0 55.5 56.3 405-986 bp 1.0 46.5