Hotspot

rpl36-rps8

LSC · 80788-80897 · 110 bp

Back to Cathetus

Pi 0.101

MarkerSeek score 77.90

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 110
variable_sites: 11
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TTTTACGAACAGAGGCCCTT	CGGGGTATAATGACAGACCG	45.0	55.0	57.1	57.6	210 bp	1.0	73.6
rpl36-rps8_p2	ACGAACAGAGGCCCTTATTT	CGGGGTATAATGACAGACCG	45.0	55.0	56.8	57.6	206 bp	1.0	71.8
rpl36-rps8_p3	ATTTTACGAACAGAGGCCCT	CGGGGTATAATGACAGACCG	45.0	55.0	56.8	57.6	211 bp	1.0	71.7
rpl36-rps8_p4	TCGTCTACGTATCAGTCGACA	CGGGGTATAATGACAGACCG	47.6	55.0	58.1	57.6	242 bp	1.0	71.5
rpl36-rps8_p5	CGTCTACGTATCAGTCGACA	CGGGGTATAATGACAGACCG	50.0	55.0	56.6	57.6	241 bp	1.0	69.6